Extremely low frequency (ELF) pulsed-gradient magnetic fields inhibit malignant tumour growth
at different biological levels
Extremely low frequency (ELF) pulsed-gradient magnetic field (with the maximum intensity of
0.6-2.0 T, gradient of 10-100 T.M(-1), pulse width of 20-200 ms and frequency of 0.16-1.34 Hz
treatment of mice can inhibit murine malignant tumour growth, as seen from analyses at different
hierarchical levels, from organism, organ, to tissue, and down to cell and macromolecules. Such
magnetic fields induce apoptosis of cancer cells, and arrest neoangiogenesis, preventing a supply
developing to the tumour. The growth of sarcomas might be amenable to such new method of
- Author: Zhang, X. and Zhang, H. and Zheng, C. and Li, C. and Xiong,
- Year: 2002
- Link: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12127939
- Comment:Zhang, Xinchen Zhang, Husheng Zheng, Congyi Li, Chaoyang Zhang,
Xinsong Xiong, Wei Research Support, Non-U.S. Gov't England Cell biology international Cell
Biol Int. 2002;26(7):599-603.
- Address: Biomedical Physics Unit, Department of Physics, Wuhan University,
Wuhan, 430072, China.
- Booktitle: Cell Biol Int
[Effects of steep pulsed electric fields on cancer cell proliferation and cell cycle]
To assess study the cytocidal and inhibitory effects of steep pulsed electric fields (SPEFs) on
ovarian cancer cell line SKOV3, the cancer cell suspension was treated by SPEFs with different
parameters (frequency, pulse duration, peak value of voltage). Viability rate and growth curves of
two test groups (high dosage and low dosage of SPEFs) and one control group were also measured. The
DNA contents and cell cycle were analyzed by flow cytometry (FCM). Different dosing levels of SPEFs
exerted obviously different effects on cancer cell viability. With the enhancement of each pulse
parameter, the viability rate was promoted and the inhibitory effect on the proliferation of
treated cells was more evident. The cells exposed to SPEFs grew slower than the control. The ratio
of S+G2/M phase cells was decreased, which restrained the DNA synthesis and division, but the ratio
of G0/G1 phase cells was increased in the treated groups. It was also indicated that the SPEFs
blocked the cell transition from G0/G1 phase to S+G2/M phase. There was a significant difference in
cell cycle between treated group and control group (P<0.01). Lethal effects of SPEFs were
represented by inhibiting the cancer cell proliferation at the cell level and by influencing the
cell cycle at the DNA level.
- Author: Yao, C. and Sun, C. and Mi, Y. and Xiong, L. and Hu, L. and Hu,
- Year: 2004
- Link: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15357428
- Comment:Yao, Chenguo Sun, Caixin Mi, Yan Xiong, Lan Hu, Lina Hu, Ya
English Abstract Research Support, Non-U.S. Gov't
- Address: Key Lab of High Voltage Engineering and Electrical New
Technology, Ministry of Education, Chongqing University, Chongqing 400044, China.
- Booktitle: Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
Effects of pulsed magnetic stimulation on tumor development and immune functions in mice
We investigated the effects of pulsed magnetic stimulation on tumor development processes and
immune functions in mice. A circular coil (inner diameter = 15 mm, outer diameter = 75 mm) was used
in the experiments. Stimulus conditions were pulse width = 238 micros, peak magnetic field = 0.25 T
(at the center of the coil), frequency = 25 pulses/s, 1,000 pulses/sample/day and magnetically
induced eddy currents in mice = 0.79-1.54 A/m(2). In an animal study, B16-BL6 melanoma model mice
were exposed to the pulsed magnetic stimulation for 16 days from the day of injection of cancer
cells. A tumor growth study revealed a significant tumor weight decrease in the stimulated group
(54% of the sham group). In a cellular study, B16-BL6 cells were also exposed to the magnetic field
(1,000 pulses/sample, and eddy currents at the bottom of the dish = 2.36-2.90 A/m(2)); however, the
magnetically induced eddy currents had no effect on cell viabilities. Cytokine production in mouse
spleens was measured to analyze the immunomodulatory effect after the pulsed magnetic stimulation.
tumor necrosis factor (TNF-alpha) production in mouse spleens was significantly activated after the
exposure of the stimulus condition described above. These results showed the first evidence of the
anti-tumor effect and immunomodulatory effects brought about by the application of repetitive
magnetic stimulation and also suggested the possible relationship between anti-tumor effects and
the increase of TNF-alpha levels caused by pulsed magnetic stimulation.
- Author: Yamaguchi, S. and Ogiue-Ikeda, M. and Sekino, M. and Ueno, S.
- Year: 2006
- Link: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16304693
- Comment:Yamaguchi, Sachiko Ogiue-Ikeda, Mari Sekino, Masaki Ueno, Shoogo
Research Support, Non-U.S. Gov't United States Bioelectromagnetics Bioelectromagnetics. 2006
- Address: Department of Biomedical Engineering, Graduate School of
Medicine, University of Tokyo, Japan. firstname.lastname@example.org
- Booktitle: Bioelectromagnetics
Therapeutic electromagnetic field effects on angiogenesis and tumor growth
BACKGROUND: A new approach to cancer therapy based on the application of therapeutic
electromagnetic fields (TEMF) has been developed by EMF Therapeutics, Inc., Chattanooga, TN, USA.
This study was designed to assess the effect of TEMF on tumor vascularization and growth of murine
16/C mammary adenocarcinoma cells in C3H/HeJ mice. MATERIALS AND METHODS: Implanted tumors were
allowed to grow for seven days until the tumor volume reached 100 mm3 before treatment was started.
Mice (20 per control, 10 per EMF exposed group) received treatment (10 minutes per day with 0, 10
mT, 15 mT or 20 mT) with a 120 pulses per second pulsating magnetic field. Tumor growth was
assessed throughout the treatment period. The extent of tumor vascularization was evaluated by
immunohistochemical staining for CD31. RESULTS: Exposure to TEMF significantly reduced tumor
growth, significantly reduced the percentage of area stained for CD31 indicating a reduction in the
extent of vascularization and there was a concomitant increase in the extent of tumor necrosis.
CONCLUSION: A novel TEMF treatment safely reduced growth and vascularization of implanted breast
cancers in mice. IMPLICATION: TEMF may prove a useful adjuvant to increase the therapeutic index of
conventional cancer therapy.
- Author: Williams, C. D. and Markov, M. S. and Hardman, W. E. and Cameron,
- Year: 2001
- Link: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11911264
- Comment:Williams, C D Markov, M S Hardman, W E Cameron, I L Greece
Anticancer research Anticancer Res. 2001 Nov-Dec;21(6A):3887-91.
- Address: EMF Therapeutics, Inc., Chattanooga, TN 37405, USA.
- Booktitle: Anticancer Res